Facs Calibur

Good Morning
I'm dr.Giuseppe Maulucci from the Catholic university Medical school of Rome. I had access to a FACScalibur citometry systems, and I'm working on
a software that allows to determine liposome size distribution with a cytofluorimeter without calibration. To this goal I need to evaluate teorethically the intensity of the radiation scattered in FS and SS directions.
I saw the instrument technical specifications at the site
http://www.bdbiosciences.com/immunocytometry_systems/brochures/FACSCalibur_specs.shtml#Sorting%20Specifications
but I need further informations about the scatter optics:
1) it is possible to get an optical scheme of the instrument?
2) the incident light radiation is polarized? the incident laser radiation is polarized perpendicular to the scattering plane?
3)There is a polarizer in front of the FS detector?
4)I see that there is an obscuration blade that limit the forward scatter radiation collection to a half-cone angle range of ~0.7 to 10 degrees. the 100-µ slit that minimize unwanted laser radiation is in front of both the FS and SS detectors? this slit is parallel to the scattering plane(i.e. perpendicular to the obscuration blade)?
5) The FS radiation is the light scattered only at 90° (optic fiber) or there is an angular spread? If so what is its magnitude?

Kind Regards


Giuseppe Maulucci