Determining Concentration from Standard Curve

Hello,

I readily admit I have a hard time with maths, so please bear with me.

My cell bio lab assayed a mitochondrial suspension and supernatant from succinate dehydrogenase (SDH). We used DCIP (dichlorophenolindophenol) to measure SDH activity using the spectrophotometer. We also used the spectrophotometer to measure absorbance via the reduction activity of DCIP without the assay (to make the standard graph).

DCIP concenentration (nanomoles) (no assay) : 0 10 20 30 40 50

Absorption: 0 .240 .490 .738 .975 1.200

Results of the SDH assay are as follows:

Time (min)/ Suspension/ Supernatant /Control

0/ 0.050 /0.109 /0.128

2/ 0.046 /0.113

4 /0.041/ 0.112

6 /0.037/ 0.111

8 /0.037/ 0.110

10 /0.034/ 0.104

12/ 0.031/0.110

14/ 0.026 /0.110 /0.130

I need to determine the DCIP concentration present at these specific time intervals for a suspension and supernatant and control sample.

I think I found the DCIP standard curve: 0.0248 nanomoles/ml

Problem: I have no idea how to proceed, what formula to use, or how the formula would work. If someone could please please explain to me a formula and how it works, I would deeply appreciate it. (I don't want someone to do my work for me...I have been trying for the past 4 days to get this down and I would like to find out HOW this works out, not just what to do...)