2.)is there any possibility to replace a two wheeler chassis in the form of 'I' section instead of a circular section or a square section please explain?
If you have to ask a public engineering blog space how to measure the distance between to points in space, STOP. You do not know what you are doing. If you want to know how to measure a two wheel frame chassis to know which size to order from a manufacturer, contact the manufacturer.
As for question 2, I always find it laughable when somebody that cannot explain their question asks others to explain their answers.
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"Don't disturb my circles." translation of Archimedes last words
Your choice of words suggests that English is not your first language - I hope that is the reason or I am wasting my time.
By "measure", I presume you mean "design" or "calculate" - the short answer for this question is: determine the loads, define the allowable stresses and deflections and from basic structural theory calculate the required cross section dimensions.
You question about the possibility of using an I section tells me that you do not understand two points:
(1) is that the frame of a 2 wheeler (motorcycle ?) has to be torsionally rigid for reasons of control and stability, and
(2) that I, U and like sections are generally unsuitable for the reason that they have low torsional rigidity and so are not easily able to be used to build torsionally rigid structures. They are called open sections, meaning that they do not enclose an area, and it is a large enclosed area that determines if a beam has high torsional rigidity.
Ifyou were to use open sections to make a motorcycle chassis it could only be as a fully triangulated frame. This would be ugly and probably hard to achieve given the other requirements of the design.
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