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Microscopic Life Sensor

09/22/2016 11:56 AM

I am looking for a "simple" way to detect if there is microscopic life in a water sample. I mean bacteria, amoebae, small eggs, and other critters. Basically anything that you cannot see easily but can make you very sic if you drink the water. I left viruses out because of their order of magnitude in size and lack of metabolism but it would be a bonus to detect them as well.

As always, the faster, the better. Can we get results in under a minute? It is basically a pass or fail test. The sensor should be resistant and unaffected by dissolved or suspended minerals since the water can be dirty. Coarse filtering is acceptable.

Ideally, automated operation would be possible.

Anybody knows a possible instrument or a combination thereof?

Thank you,

Marco

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#1

Re: Microscopic life sensor

09/22/2016 11:59 AM

Anything from a bench-top microscope to a Total Organic Carbon analyser will fit the bill, Mildred.

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#3
In reply to #1

Re: Microscopic life sensor

09/22/2016 12:11 PM

Very good!

It seems to be a candidate.

Thank you!

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#2

Re: Microscopic life sensor

09/22/2016 12:02 PM

Heat your water to 161°F for 30 minutes and call me in the morning....

http://newatlas.com/bacteria-water-sensor/39794/

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#4
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Re: Microscopic life sensor

09/22/2016 12:14 PM

That also could work.

Thank you!

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#5
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Re: Microscopic life sensor

09/22/2016 12:20 PM
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#6

Re: Microscopic life sensor

09/22/2016 12:33 PM

It sounds as if this is untreated surface water.

If yes, I would believe that it is almost a certainty that microscopic life - bacteria, amoebae, small eggs, and other critters - are in the sample.

If no organisms are present, I believe it is a certainty the sample is unfit for human consumption.

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#7

Re: Microscopic Life Sensor

09/22/2016 1:26 PM
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#8
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Re: Microscopic Life Sensor

09/22/2016 1:52 PM

Yes, really fancy. Not real time yet though.

Thank you

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#9

Re: Microscopic Life Sensor

09/22/2016 2:40 PM

Sorry can't resist the self referential play on words:-

"can make you very sic if you drink the water." (sic.?)

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#10
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Re: Microscopic Life Sensor

09/22/2016 3:11 PM

Excuse my "sick" English. I learned it later in life. "Sic" sounded good though

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#11

Re: Microscopic Life Sensor

09/22/2016 3:27 PM

A small microscope shows tiny critters swimming around in "swamp water".

Maybe a video microscope connected to a computer and software which would take a picture periodically and compare successive shots. It should trigger when "something" swims by.

Sounds like an interesting project.

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#14
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Re: Microscopic Life Sensor

09/22/2016 3:55 PM

There might be video security software out there that has "motion detection" that might be useful for this application.

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#15
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Re: Microscopic Life Sensor

09/22/2016 4:00 PM

Not sure how many amoeba will make aggressive motions resembling a home invasion squad, or a burglar.

No need to re-invent the wheel on this one, it has been invented already.

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#20
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Re: Microscopic Life Sensor

09/23/2016 9:24 AM

Good idea

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#12

Re: Microscopic Life Sensor

09/22/2016 3:46 PM

As Solar Eagle pointed out, Electric Conductivity Flow Cytometry (from a Danish Company) can count and distinguish bacteria from other organic matter and inorganic particulates in the water, in a continuous, automated system. Total Organic Carbon may correlate somewhat with bacterial colonization in waters, however, it is not a conclusive test at all, more of a crude indicator, and it requires some skill as a test operator for reliable results.

ATP testers that rely upon the reaction of luciferase enzyme with ATP (the energy carrier in living organisms, such as bacteria (not necessarily viruses). The resulting luminescence signal is proportional to the bacterial ATP present, and the test can be made to distinguish between ATP in solution (extracellular) and within the cells.

Microscopy is only done when key pathogenic organisms are suspected, cryptosporidum for example. Phase contrast microscope may produce better diagnostic images in some situations.

Older instruments rely upon tube with culture media in them, and the tube tip is broken under water to draw in the sample. Tube then is incubated for a period of time, this depends on the type or class of bacteria being sought.

Cheapest alternative: If you are dealing with a stagnant, or even flowing water source, you do not know if something died upstream, or is submerged. Certain algal blooms even in fresh water can disrupt the quality of the water and emit toxins that are among the most toxic in the biosphere. If there are dead fish, leave the water alone. You do not have the skill set (most likely) to remediate this water to drinkable status. Most waters without strong odors, large amounts of suspended matter, or odd colors can be boiled for about 20-30 minutes to render these safe. Even better would be to distill this water, then contact it with limestone that has been heated to at least 200 °C to kill organisms. The limestone will produce a dilute solution of calcium carbonate that is near saturation, so the water is less likely to leach your minerals, if that is even a concern.

You can buy so-called drinking straws that will make pretty nasty water reasonably safe to drink, but I would avoid slimy waters, as this can plug up the device quickly.

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#21
In reply to #12

Re: Microscopic Life Sensor

09/23/2016 9:31 AM

Thank you.

As we get more answers, we see that it is not a simple problem...

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#13

Re: Microscopic Life Sensor

09/22/2016 3:52 PM

Don't drink water that isn't potable.

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#16

Re: Microscopic Life Sensor

09/22/2016 5:55 PM

If I don't know for sure that the source is "OK", these seem to work well.

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#17

Re: Microscopic Life Sensor

09/22/2016 11:47 PM

Logic and eyes are your best sensor.

Logic dictates that there always is and will be bacteria.

Eyes can tell you if you at least are holding a bottle of distilled water in hand.

If you ask me I was not even sure if distilled water would be free of bacteria.

They cling to the bottle wall like spider silk to hair.

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#18

Re: Microscopic Life Sensor

09/23/2016 6:43 AM

I'm sure that HACH and other equipment suppliers could provide a "Total Organic Carbon" test device with sequence time in the order of 1 minute. This will tell you whether carbon based material is present, but not whether it is curently "alive" at the time of testing.

This would give you a go/nogo outcome.

In the water processing indusry, current best practice for pathogens is a 24 hour incubation process using multiple cells and a nutrient additive to enhance organism growth.

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#19
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Re: Microscopic Life Sensor

09/23/2016 8:39 AM

TOC is no indicator of potable or not potable. You could get a low TOC reading, and still have pathogen organism present that will kill you.

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#23
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Re: Microscopic Life Sensor

09/24/2016 4:09 AM

This would depent on what they selected as their go/nogo decission point.

I'm suggesting a TOC that may be acceptable in water with residual chlorine and other known treatments would NOT be the threshold used in such a review for water of unknown origin.

TOC could also indicate the presence of some toxins from previously living organisms that would not show in culture or microscope examination.

You and I both know that he really needs a treatment system with independant barriers, backed up by an analysis system that is planned and verified. He may not have the resources to move that far from his curent situation and is at least looking to move to an improved situation.

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#24
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Re: Microscopic Life Sensor

09/24/2016 3:19 PM

In that case, he does not need a sensor, he needs chlorine, and a lot of heat. Boil it and be relatively safe.

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#25
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Re: Microscopic Life Sensor

09/25/2016 4:08 AM

That's right, and it works both ways. A cup of tea with milk has high TOC, COD, BOD etc but won't kill you!

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#22

Re: Microscopic Life Sensor

09/23/2016 10:44 AM

The good the bad and the ugly.....

http://extoxnet.orst.edu/faqs/safedrink/microorg.htm

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