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Location: Monchengladbach, Germany
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DNA testing.

11/01/2009 5:46 AM

This may be a stupid question, but how do people do like paternity tests using blood, when blood cells don't have any DNA cos they don't have a nucleus or any organelles?

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#1

Re: DNA testing.

11/01/2009 5:54 AM

apparently, a blood test for paternity testing and DNA paternity testing are 2 different procedures. but, indeed DNA can be found in white blood cells. From that previous link:

"Most people know that you can recover DNA from blood, but few people know that Red Blood Cells (RBC's), the cells that make blood red, do not contain DNA. DNA is found in the nucleus of the cell and RBC's don't have a nucleus! DNA is actually recovered from the White Blood Cells in blood."

please see the following (of several) references for both:

HTH!

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#3
In reply to #1

Re: DNA testing.

11/02/2009 12:42 AM

Immature RBC's do have a nucleus while they're in the marrow, but lose it before they move into circulation. So what you say is correct for DNA testing purposes.

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#2

Re: DNA testing.

11/01/2009 8:38 AM

There are two types of Paternity Tests: DNA and Blood Type tests. The first DNA test used for legal purposes was 1986, so before then only blood typing paternity tests were used. DNA tests can be done by swabbing the mouth for cells or by blood cells. Blood, as others has mentioned has WBC's and platelets which has DNA. Also, reticulocytes, which are new RBC's do not have a nucleus, but, usually still have the mitochondria. Mitochondrial DNA could be tested. Mitochondrial DNA is only from the mother not the father.

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#4

Re: DNA testing.

11/02/2009 11:36 AM

Definitely not a stupid question!

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Anonymous Poster
#5

Re: DNA testing.

11/02/2009 2:14 PM

This is absolutely not a stupid question, I am looking forward to the responses because I know absolutely nothing about this subject. Thanks for a great question.

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#6

Re: DNA testing.

11/03/2009 2:40 AM

Ally: Blood contains RBC's and WBC's...white blood cells (WBC's) do contain DNA.

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#7

Re: DNA testing.

11/13/2009 2:16 AM

Blood is the most commonly used source of DNA for testing. Blood should be collected in a purple top tube or one containing EDTA preservative which inhibits DNA degradation during shipment. A few labs now accept blood spotted onto filter paper. Blood may be refrigerated but many labs ask that it not be frozen because this lyses all the cells and only white blood cells contain DNA in mammals. The first generation of parentage testing was based on blood typing (blood groups and protein polymorphisms), using a series of reliable, low-cost and technologically simple testing routines, now provided by laboratories worldwide. While these tests have been highly effective in providing solutions to paternity questions and sorting out switched foal scenarios, three limitations have occasionally, but repeatedly, provided frustrations. First, the tests can only be performed with fresh blood, thus excluding the possibility to use other kinds of samples, especially those that might be available from dead. Second, the requirement of a blood sample may necessitate the assistance (and expense) of a specialist, special shipping containers, handling conditions and difficulties in the international transport of samples. Third, if a solution could not be obtained from the available blood typing tests, no other effective tests were available to extend the test battery.

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